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KMID : 0861020220370060009
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Korea Journal of Herbology
2022 Volume.37 No. 6 p.9 ~ p.17
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Protective effect of Saenggangeonbi-tang on liver fibrosis induced by thioacetamide
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Choi Jeong-Won
Shin Mi-Rae
Jeong Da-Un
Roh Seong-Soo
Chung Sung-Mi
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Abstract
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Objective : In modern society, liver diseases such as liver fibrosis are on the rise as inflammation and wound healing
processes of the liver are repeated due to factors such as drinking, smoking, and stress. This study was conducted
to evaluate the effect of Saenggangeonbi-tang (SGGBT) on thioacetamide (TAA)-induced liver fibrosis.
Methods : The mice were divided into 4 groups for examination (n=6): Normal group (Nor), distilled water-treated
liver fibrosis mice (Con), silymarin 50 mg/kg-treated liver fibrosis mice (Sily), SGGBT 200 mg/kg-treated liver
fibrosis mice (S200). Liver fibrosis was established in the mice via TAA for 8 weeks (1 week 100 mg/kg, 2,3 weeks
200 mg/kg, 4-8 weeks 400 mg/kg, three times a week, intraperitoneal injection) and they were administered silymarin
and SGGBT (every day, oral administration) with the TAA.
Results : SGGBT significantly decreased the levels of aspartate aminotransferase, alanine aminotransferanse, ammonia,
and myeloperoxidase in serum increased by liver fibrosis. As a result of confirming H&E and MT staining, it was confirmed that SGGBT reduced damage and inflammatory cell infiltration in liver tissue, and alleviated changes in
collagen fiber deposition and histological fibrosis. Also, it was confirmed through PAS staining that it reduced glycogen
deposition in liver tissue. In addition, SGGBT significantly decreased the NADPH oxidases as well as significantly
modulated the expression of MMP-2 and TIMP-2.
Conclusions : These results suggest that SGGBT regulates the expression of MMP/TIMP protein through inhibition
of oxidative stress and alleviates liver fibrosis by reducing collagen and glycogen deposition in liver tissue.
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KEYWORD
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Liver fibrosis, Saenggangeonbi-tang, thioacetamide, histological analysis
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